Exercise
1. Obtain the videotape labeled
“Aseptic technique” from the Tech Facility instructor and view it.
When finished, rewind it in the tape rewinder and return it to the
instructor.
Exercise
2. Obtain the box labeled
“Aseptic technique” from the Tech Facility instructor, and examine its
contents. In it you will find:
(a)
a bunsen burner (DO NOT TRY TO ATTACH IT TO THE GAS)
(b)
an inoculation loop and needle
(c)
a sterile pipette
(d)
a capped erlenmeyer flask containing “medium”
(e)
a tube rack with “sterile” capped tubes, some containing “cultures” and
others containing “media” (see labels)
(f)
a Petri dish containing an agar-based medium
(g)
a bottle of “disinfectant”
Exercise
3. Flaming the loop and inoculating
a liquid culture
(a)
Use a paper towel and some of the “disinfectant” to decontaminate your
desktop work area.
(b)
Plug the bunsen burner pump into an electrical outlet!
This will produce a simulated “flame”.
(c)
Use the loop to remove a sample from one of the capped tubes labeled
“culture”, following the video procedure.
(d)
Transfer the loop sample into a “medium” tube (remember to “flame” the
second tube, and flame the loop when finished)
Exercise
4. Flaming the loop and inoculating
a semi-solid medium in a Petri dish
(a) Plug the bunsen burner pump into an electrical outlet if not already plugged
in! This will produce a simulated
“flame”.
(b)
Use the loop to remove a sample from one of the capped tubes labeled
“culture”, following the video procedure.
(c)
Transfer the loop sample into a Petri dish, streaking the plate.
Remember to keep the Petri dish lid only slightly open, and above the
dish bottom when working, as in the video. Flame the loop when finished.
Exercise
5. Transferring medium by pipette.
(a) Obtain the erlenmeyer flask,a sterile pipette, and one of the capped tubes.
(b)
Use the pipette plus a pipette bulb (or other device) to transfer 2 ml of medium
from the erlenmeyer flask to a culture tube.
Remember to “flame’ both the flask and the tube, as in the video.
(c)
Dispose of the pipette in the special waste container (red bag - biohazard) near
the work area
FINALLY, CLEAN UP YOUR WORK AREA; PLEASE PUT ALL ITEMS BACK IN THE ORIGINAL BOX AND RETURN THE BOX TO ITS STORAGE PLACE.